Activity profiling of ectomycorrhiza communities in two forest soils using multiple enzymatic tests.
نویسندگان
چکیده
Data on the diversity and distribution of enzyme activities in native ectomycorrhizal (ECM) communities are inadequate. A microplate multiple enzymatic test was developed which makes it possible to measure eight enzyme activities on 14 individual, excised ECM root tips. Hydrolytic and oxidative enzymes are involved in the decomposition of lignocellulose, chitin and phosphorus-containing organic compounds. This test system was used to describe the functional diversity of ECM communities in two forest sites. This set of tests proved to be accurate and sensitive enough to reveal a high diversity of activity profiles, depending on the fungal symbiont and the soil horizon. Ectomycorrhizas can be classified into specialists and generalists, and appear to complement each other in the same horizon to collectively perform all eight activities studied. By including a higher number of different assays for more detailed analyses, ECM activity profiling will provide a valuable tool for studying the functional diversity of ECM communities.
منابع مشابه
Fungal and bacterial communities across meadow–forest ecotones in the western Cascades of Oregon
Meadows are natural dynamic features of forested mountain landscapes of the Pacific Northwest. Proportions of meadows and forests change with environmental conditions and disturbance history. We investigated the belowground microbial communities associated with these two vegetation types and how they change across the meadow–forest transition at two sites in Oregon. Soils were sampled along rep...
متن کاملComparison of diversities and compositions of bacterial populations inhabiting natural forest soils.
The diversity and composition of soil bacterial communities were compared among six Austrian natural forests, including oak-hornbeam, spruce-fir-beech, and Austrian pine forests, using terminal restriction fragment length polymorphism (T-RFLP, or TRF) analysis and sequence analysis of 16S rRNA genes. The forests studied differ greatly in soil chemical characteristics, microbial biomass, and nut...
متن کاملMicrobial Community Structure and Function of Soil Following Ecosystem Conversion from Native Forests to Teak Plantation Forests
Soil microbial communities can form links between forest trees and functioning of forest soils, yet the impacts of converting diverse native forests to monoculture plantations on soil microbial communities are limited. This study tested the hypothesis that conversion from a diverse native to monoculture ecosystem would be paralleled by a reduction in the diversity of the soil microbial communit...
متن کاملAnaerobic Decomposition of Switchgrass by Tropical Soil-Derived Feedstock-Adapted Consortia
UNLABELLED Tropical forest soils decompose litter rapidly with frequent episodes of anoxic conditions, making it likely that bacteria using alternate terminal electron acceptors (TEAs) play a large role in decomposition. This makes these soils useful templates for improving biofuel production. To investigate how TEAs affect decomposition, we cultivated feedstock-adapted consortia (FACs) derived...
متن کاملDistinctive fungal and bacterial communities are associated with mats formed by ectomycorrhizal fungi
The distinct rhizomorphic mats formed by ectomycorrhizal Piloderma fungi are common features of the organic soil horizons of coniferous forests of the Pacific Northwest. These mats have been found to cover 25e40% of the forest floor in some Douglas-fir stands, and are associated with physical and biochemical properties that distinguish them from the surrounding non-mat soils. In this study, we ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The New phytologist
دوره 167 1 شماره
صفحات -
تاریخ انتشار 2005